Hemostatic and phytochemical testing of “Peley”

Eunice A. Carpio  |  Precious Mae B. Esmeralda
Princess Jeanly R. Rañeses  |  Marilyn M. Tomilas
School of Natural Sciences
Mrs. Teresa N. Villanueva, Adviser


“Peley” is a common name of a plant which belongs to a family of trees described as trifoliate and grows abundantly in Baw-ek, Taloy Sur. This study evaluated the hemostatic effect of “Peley” extract by determining the in vitro coagulation of venous blood. The “Peley” leaves were submitted to the Department of Science and Technology at La Trinidad, Benguet for phytochemical analysis to determine its which contributes to its healing effect. Phytochemical analysis results showed that the “Peley” Leaves are positive for alkaloids, tannins, flavonoids, and saponins. Venous blood samples were collected from 10 individuals and were treated with 100 microliters of the plant extract. The Serum Separator Tube (SST) was used as the control for the experiment. The venous blood was collected in a tube which contained the extract.  The time of coagulation was recorded and compared to venous blood collected in SST to assess the hemostatic effect of the plant extract. The results of the clotting time determination showed that the venous blood collected in test tubes containing the “Peley” extract had shorter clotting time compared to blood collected in SST.  It was determined using T-Test that there was no significant difference in the clotting time of venous blood admixed with the “Peley” extract and blood in SST. It is thereby concluded that the “Peley” plant promotes coagulation of blood due to the presence of tannins which contributes to the plant’s ability to heal wounds. The plant may also be used as an alternative agent in promoting in vitro clotting.

Key words:  Phytochemical analysis, hemostatic effect, Peley, coagulation, Baw-ek,
Taloy Sur